Abstract:Fever is the most common disease in children. Fever in children can be found in various conditions such as infection, inflammation, or other unknown causes. One of the laboratory tests that can be used is the hs-CRP and…
erythrocyte sedimentation rate examination. The purpose of this study was to determine the relationship between hs-CRP levels and erythrocyte sedimentation rate in pediatric febrile patients. The research method was observational with a cross-sectional method using secondary data of 62 samples. Data analysis used the Spearman's rho test. The results of the study showed that most of the pediatric patients who experienced fever were male (59.7%), and the age of the child was 5 years (21%). In pediatric patients with fever, the average hs-CRP level was 19.34 mg/l, and the erythrocyte sedimentation rate was 32.73 mm/hour. The results of the correlation test using the Spearman's rho test method obtained p = 0.001 with r = 0.575, so it can be concluded that there is a moderate and positive relationship between hs-CRP levels and erythrocyte sedimentation rate in pediatric patients with fever.
Abstract:Urine alcohol testing plays a vital role in detecting alcohol consumption for clinical and forensic purposes, yet its results may be influenced by pre-analytical factors such as the delay between sample collection and laboratory…
boratory analysis. This study aimed to evaluate the effect of delay time before urine examination on alcohol concentration. An experimental One-Shot Case Study design was conducted involving 44 participants in Denpasar who had consumed alcohol. Alcohol concentration was measured immediately, after a 2-hour delay, and after a 4-hour delay using the Alcohol Urine Rapid Test Dipstick Allchek, and the data were analyzed using the Kruskal-Wallis test. The findings showed that average urine alcohol levels increased numerically after a 2- and 4-hour delay compared to immediate testing; however, this change was not statistically significant (p=0.131). These results confirm that delaying urine alcohol examination up to 4 hours does not significantly alter detectable alcohol concentrations, although prompt testing is still recommended to ensure result accuracy and minimize the risk of false positives. This study provides important insights for the development of standard operating procedures regarding urine alcohol analysis in laboratory practice.
Abstract:Dengue Hemorrhagic Fever (DHF) is a disease caused by the Dengue virus transmitted to humans through the bite of the Aedes aegypti and Aedes albocpitus mosquitoes. One of the laboratory tests that can help diagnose Dengue…
e fever includes Dengue IgM and IgG Antigens and hematology tests. The number of lymphocytes plays a role in the body's immune system and is one of the parameters found in hematology tests. This study aims to determine the relationship between Dengue IgM and IgG antibodies and the number of lymphocytes in patients suspected of having Dengue fever at the Prodia Sunter Clinical Laboratory for the period 2021-2024. The research method is observational with cross-sectional using secondary data of 104 samples. Data analysis uses Spearman rank. The results of the study showed that most of the suspected Dengue fever patients were male, 56 patients (53.8%) with the highest age at 19-59 years as many as 53 patients (51.0%) and the duration of fever was mostly experienced on the 3rd day of fever 29 patients (27.9%). The results of the Dengue antigen IgM examination in suspected Dengue fever patients were negative for 89 patients (85.6%) and the Dengue antigen IgG was negative for 64 patients (61.5%). The number of lymphocytes was within normal limits for 44 patients (42.3%). The results of the IgM correlation test obtained p = 0.005 with r 0.271, so the hypothesis was accepted, it can be concluded that there is a weak correlation between Dengue IgM antibodies and the number of lymphocytes and the results of the IgG correlation test obtained p = 0.043 with r 0.198, so the hypothesis was accepted, it can be concluded that there is a weak correlation between Dengue IgG antibodies and the number of lymphocytes in suspected Dengue fever patients.
Abstract:Cleaning service staff working in laboratories are at high risk of exposure to hazardous materials. The use of personal protective equipment (PPE) is a crucial step in preventing occupational accidents. However, the level…
l of compliance with PPE usage varies and is influenced by the knowledge and attitude of the staff. This issue highlights the need to understand the relationship between the knowledge and attitudes of cleaning service staff and their compliance with the use of PPE. This study employed an analytical design with a cross-sectional approach. A total of 58 cleaning service staff participated as respondents, selected through total sampling. Data were collected using a structured questionnaire and analyzed using the chi-square test. The results showed that 60.3% of respondents had good knowledge regarding the use of PPE, while 73% had a positive attitude toward its usage. The chi-square test revealed a p-value of 0.03 (<0.05), indicating a significant relationship between attitude and compliance with PPE usage among cleaning service staff. The chi-square test for the relationship between knowledge and compliance yielded a p-value of 0.01 (<0.05), indicating a significant relationship between knowledge and compliance. The findings indicate that the knowledge of cleaning service staff positively correlates with their compliance in using PPE. Staff members with good knowledge were more likely to comply with PPE usage. Adequate knowledge enables staff to understand laboratory risks and the benefits of PPE in preventing exposure to hazardous materials, thereby encouraging safer behavior, promoting communication among staff, reminding one another, and embedding safety values into daily work routines.
Abstract:Congenital hypothyroidism (CH) is an endocrine disorder that can cause developmental delays if not detected and treated early. Congenital Hypothyroidism Screening (CHS) aims to detect abnormal levels of Thyroid Stimulating…
ng Hormone (TSH) in newborns before clinical symptoms appear. The timing of blood sample collection is a critical factor because TSH levels physiologically surge within the first 24 hours of life. This study aims to determine the accuracy of TSH sampling time in CH screening at Prima Medika General Hospital, Denpasar. A descriptive analytical method was used with a total sampling technique involving 105 neonates who underwent CHS from October to December 2024. Secondary data were obtained from laboratory request forms and the Laboratory Information System (LIS). Results showed that 92.4% of samples were collected on the second day of life, which aligns with the ideal window of 48–72 hours, and only 1.9% of neonates had elevated TSH levels. The study concludes that most sample collections met the recommended timing, contributing to valid results and effective CHS implementation. Timely sampling plays a vital role in early CH detection and reflects the overall quality of neonatal laboratory services.
Abstract:Dengue Hemorrhagic Fever (DHF) is a viral infection marked by a significant decrease in platelet count. Accurate platelet count testing is crucial for diagnosing and monitoring DHF patients. The pre-analytical phase is a…
critical stage in laboratory testing, where errors can significantly impact test outcomes. Anticoagulants, such as K3EDTA and 3.2% sodium citrate, are commonly used in routine hematology tests to prevent blood clotting by binding calcium ions and inhibiting clotting factor activation. This study aimed to evaluate the difference in platelet counts between blood samples collected with K3EDTA and 3.2% sodium citrate anticoagulants in DHF patients. An analytical observational study with a cross-sectional design was conducted involving 50 purposively sampled DHF patients. Statistical analysis yielded a p-value of 0.615 (p > 0.05), indicating no significant difference in platelet counts between the two anticoagulants. It can be concluded that the use of K3EDTA and 3.2% sodium citrate does not significantly affect platelet count results in DHF patients. Future studies are recommended to involve larger sample sizes and consider microscopic examination methods, such as peripheral blood smears, to assess platelet aggregation visually.
Abstract:Cardiovascular disease remains a leading cause of death both globally and nationally, with Low Density Lipoprotein(LDL) levels serving as a key biochemical indicator in the screening and management of heart disease. Accurate…
rate LDL testing requires adherence to pre-analytical procedures, particularly fasting for 10-12 hours. This study aimed to describe LDL levels and fasting compliance among outpatient cardiac patients at RSU Prima Medika Denpasar. A descriptive cross-sectional design was used with purposive sampling, involving 35 cardiac outpatients from February to March 2025. Data were collected through interviews and laboratory examinations using the Furuno CA-270 device. Results showed that 71% of respondents had LDL levels >100 mg/dL, indicating high levels, while 29% hadnormal levels (<100 mg/dL). All respondents (100%) complied with the fasting requirement. These findings indicate that high LDL levels are still commonly found among outpatient cardiac patients, particularly in productive-age males. The high level of fasting compliance reflects the effectiveness of pre-analytical patient education. This study emphasizes the importance of routine lipid screening and continuous education to enhance laboratory diagnostic accuracy and prevent cardiovascular complications.
Abstract:Healthcare services must continuously be improved to provide the best care for the community. Patient satisfaction is the result of patients' evaluations of healthcare services, comparing their expectations with the actual…
al healthcare services received in a hospital setting. Patient satisfaction with laboratory services is not solely determined by the availability of sufficient healthcare personnel but also requires strong commitment and dedication from healthcare workers to provide professional services, supported by adequate facilities and medical equipment. This study aims to assess the level of outpatient satisfaction with laboratory services at RSUD Klungkung. The research employs an observational method with a cross-sectional design. Data collection was conducted using questionnaires, with a sample of 97 respondents selected through purposive sampling. The data analysis in this study was performed using the Chi-Square test. Based on the five service quality dimensions—reliability, responsiveness, assurance, empathy, and tangibles—the results showed a p-value < 0.05. Among these dimensions, responsiveness had the lowest p-value (0.005 < 0.05), indicating its stronger influence. These findings conclude that there is a significant relationship between service quality dimensions and the level of outpatient satisfaction with laboratory services at RSUD Klungkung. Furthermore, the responsiveness dimension, which had the lowest p-value compared to the other four dimensions, indicates that it has the most significant impact on patient satisfaction with laboratory services provided by the staff at RSUD Klungkung.
Abstract:Mangrove snails (Telescopium telescopium ) are a type of gastropod that live in brackish water or mangrove forests dominated by mangrove plants. Mangrove snails have a fairly high nutritional content consisting of fat, protein,…
rotein, essential amino acids, and saturated fatty acids. This study aims to determine the fat content of mangrove snails from two different locations, namely in the mangrove forest waters of Negeri Luhu and the mangrove forest waters of Waai Village. This type of research is descriptive qualitative with the object of research being the fat content of mangrove snails. This research was conducted at the Basic Chemistry Laboratory of Pattimura University, Ambon, which was carried out starting on October 14, 2020. Data collection was obtained from the results of the analysis of the fat content of mangrove snails using the soxhlet method. The results of the study showed that from six samples of mangrove snails obtained from two different locations, it was known that the mangrove snail samples in Negeri Luhu had a higher fat content with an average value of 4.5448% compared to the mangrove snail samples in Waai Village which had an average value of 3.3649 %.
Abstract:This research evaluates the performance of coastal safety structures in mitigating wave runoff through laboratory-scale simulations. Pancana Beach in Barru Regency, South Sulawesi, was selected as the research site due to…
o its vulnerability to wave-induced beach damage. To simulate wave conditions, a wave generator was designed using a 12-volt DC motor integrated into an acrylic wave bath. This research analyses the effectiveness of three types of coastal safety structures: reflectors, sea walls and sloping sea walls. Laboratory experiments measured the wave overflow volume for each type of structure under controlled conditions. The results showed that reflector-type structures achieved the highest efficiency in mitigating wave overtopping, with an average minimum overtopping value of 0.006 m³/ms. The findings emphasise the importance of selecting and designing appropriate coastal protection measures to minimise coastal erosion, protect coastal infrastructure and reduce risks to surrounding settlements. This study contributes valuable insights into the role of engineered structures in enhancing coastal resilience, especially for areas vulnerable to high wave energy.