Showing 2 articles found for "Coli"
Journal Of Global Research Publications (Vol. 2, No. 1, 2025)

Wastewater Quality Test Using The MPN (Most Probable Number) Method To Detecte Total Coliform Bacteria

Ayu, Alfiana, Gultom, Endang Sulistyarini
Abstract: This study aims to Test Wastewater Quality Using the Mpn (Most Probable Number) Method to Detect Total COLIFORM Bacteria. This study uses laboratory analysis, which aims to provide an overview of the microbiological quality… ity of wastewater based on the presence and number of Coliform bacteria. The working principle of the Most Probable Number (MPN) method in detecting total Coliform bacteria in wastewater is carried out semi-quantitatively by detecting microbial growth through lactose fermentation which produces gas or turbidity in selective liquid media. The implementation procedure includes a presumptive test stage using Lactose Broth (LB) media, a confirmatory test with Brilliant Green Lactose Bile Broth (BGLB) media, and determining the final value by matching the combination of positive tubes with the standard MPN table. From the results of laboratory testing on three domestic wastewater samples (1224 AL, 1225 AL, and 1226 AL), it was found that all samples showed negative results with a combination of 0-0-0 tubes in the presumptive test. This indicates that the tested sample does not contain Total Coliform bacteria or has a value of less than 1000 MPN/100 mL, so that its microbiological quality is declared very good and has met the quality standard threshold set by the government through the Minister of Environment Regulation No. 5 of 2014 and PP RI No. 22 of 2021
Nusantara Research Wastewater Quality Test Using Most Probable Number Method Detecte Total Coliform Bacteria
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Jurnal Dunia Ilmu Kesehatan (JURDIKES) (Vol. 1, No. 1, 2023)

Gambaran Bakteri Escherichia Coli Pada Jajanan Gorengan Di Sepanjang Jalan Tlogosari Raya Semarang

Septiyasari, Errina, Sofyanita, Eko Naning
Abstract: Gorengan merupakan makanan yang digoreng menggunakan minyak. Lokasi pedagang gorengan di sepanjang Jalan Tlogosari Raya Semarang yang berada di pinggir jalan dan kurangnya perhatian pedagang terhadap kebersihan dapat menimbulkan… imbulkan kontaminasi kuman terhadap gorengan. Salah satu bakteri yang potensial mencemari makanan adalah Escherichia coli.  E coli merupakan flora normal berbentuk batang gram negatif di dalam saluran pencernaan hewan dan manusia. Bakteri E coli  dapat berasifat patogen jika jumlah bakteri dalam saluran pencernaan meningkat atau berada di luar usus. Penelitian ini merupakan penelitian observasional dengan kriteria penelitian deskriptif melalui pendekatan cross sectional. Hasil Penelitian yang didapat Dari 7 sampel yang diperiksa, sebanyak 1 sampel (14,29%) tercemar bakteri Escherichia coli, dan 6 sampel (75%) lainnya tidak tecemar  bakteri Escherichia coli. Satu sampel tersebut tidak sesuai dengan Peraturan Menteri Kesehatan Republik Indonesia No. 1096/Menkes/Per/VI/2011 karena mengandung bakteri Escherichia coli > 0/gram sampel makanan. Dari hasil observasi didapatkan sebanyak 1 sampel (14,29%) pedagang gorengan yang mempunyai kriteria tingkan hygiene dan sanitasi yang baik. Sedangkan 6 sampel (85,71%) lainnya memiliki kriteria tingkat hygiene dan sanitasi yang sedang.
Nusantara Research Gambaran Bakteri Escherichia Coli Pada Jajanan Gorengan Sepanjang Jalan Tlogosari Raya Semarang
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